Article
Combined Pan-RAF and MEK Inhibition Overcomes Multiple Resistance Mechanisms to Selective RAF Inhibitors.
Molecular cancer therapeutics - 1 Dec 2015
Whittaker Steven R, Cowley Glenn S, Wagner Steve, Luo Flora, Root David E, Garraway Levi A
Abstract excerpt
RAF and MEK inhibitors are effective in BRAF-mutant melanoma but not in BRAF-mutant colorectal cancer. To gain additional insights into this difference, we performed a genome-scale pooled shRNA enhancer screen in a BRAF-mutant, RAF inhibitor-resistant colorectal cancer cell line exposed to the selective RAF inhibitor PLX4720. We identified multiple genes along the receptor tyrosine kinase (RTK)/mitogen-activated...
Topics
- Apoptosis
- Cell Line, Tumor
- Colorectal Neoplasms
- Drug Resistance, Neoplasm
- Humans
- Indoles
- MAP Kinase Kinase Kinases
- MAP Kinase Signaling System
- Mutation
- Protein Kinase Inhibitors