Article
Genotyping and Classification of Tunisian Strains of Avian Reovirus using RT-PCR and RFLP Analysis.
Avian diseases - 1 Mar 2015
Kort Ymene Hellal, Bourogâa Hager, Gribaa Latifa, Hassen Jihene, Ghram Abdelgelil
Abstract excerpt
Since 1998, avian reovirus (ARV) infection has been detected in broiler and breeding chicken flocks in Tunisia. The genotype of avian reoviruses was established using simple and rapid approaches. Reverse transcription PCR (RT-PCR) on both sigma C (σC) and sigma B (σB)-encoding genes followed by restriction fragment length polymorphism (RFLP) analyses were used to better characterize Tunisian isolated strains. The...
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