Article
An improved cucumber mosaic virus-based vector for efficient decoying of plant microRNAs.
Scientific reports - 17 Aug 2015
Liao Qiansheng, Tu Yifei, Carr John P, Du Zhiyou
Abstract excerpt
We previously devised a cucumber mosaic virus (CMV)-based vector system carrying microRNA target mimic sequences for analysis of microRNA function in Arabidopsis thaliana. We describe an improved version in which target mimic cloning is achieved by annealing two partly-overlapping complementary DNA oligonucleotides for insertion into an infectious clone of CMV RNA3 (LS strain) fused to the cauliflower mosaic...
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