Article
UDP-glucose pyrophosphorylase influences polysaccharide synthesis, cell wall components, and hyphal branching in Ganoderma lucidum via regulation of the balance between glucose-1-phosphate and UDP-glucose.
Fungal genetics and biology : FG & B - 1 Sept 2015
Li Mengjiao, Chen Tianxi, Gao Tan, Miao Zhigang, Jiang Ailiang, Shi Liang, Ren Ang, Zhao Mingwen
Abstract excerpt
UDP-glucose pyrophosphorylase (UGP) is a key enzyme involved in carbohydrate metabolism, but there are few studies on the functions of this enzyme in fungi. The ugp gene of Ganoderma lucidum was cloned, and enzyme kinetic parameters of the UGP recombinant protein were determined in vitro, revealing that this protein was functional and catalyzed the reversible conversion between Glc-1-P and UDP-Glc. ugp silencing...
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