Article
Analysis of genetic and aflatoxin diversity among Aspergillus flavus isolates collected from sorghum seeds.
Journal of basic microbiology - 1 Nov 2015
Divakara S T, Aiyaz M, Moore G G, Venkataramana M, Hariprasad P, Nayaka S Chandra, Niranjana S R
Abstract excerpt
Thirty-four Aspergillus flavus isolates were recovered from sorghum seeds sampled across five states in India. Our study included (1) species confirmation through PCR assay, (2) quantification of total aflatoxin concentrations by the indirect competitive-ELISA (ic-ELISA) method, and (3) analysis of molecular diversity among the A. flavus isolates using β-tubulin, ITS, and ISSR markers. Among the isolates studied,...
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