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Article

Lepr(db) mouse model of type 2 diabetes: pancreatic islet isolation and live-cell 2-photon imaging of intact islets.

Journal of visualized experiments : JoVE - 11 May 2015

Do Oanh H, Low Jiun T, Thorn Peter

Abstract excerpt

Type 2 diabetes is a chronic disease affecting 382 million people in 2013, and is expected to rise to 592 million by 2035 (1). During the past 2 decades, the role of beta-cell dysfunction in type 2 diabetes has been clearly established (2). Research progress has required methods for the isolation of pancreatic islets. The protocol of the islet isolation presented here shares many common steps with protocols from...

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Share to Diabetes Mellitus, Experimental
Share to Diabetes Mellitus, Type 2

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Insulin

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Share to Receptors, Leptin

Source

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Identifiers

PMID: 25992768
DOI: 10.3791/52632
PMCID: PMC4542571

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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