Article
Functional profiling in Streptococcus mutans: construction and examination of a genomic collection of gene deletion mutants.
Molecular oral microbiology - 1 Dec 2015
Quivey R G, Grayhack E J, Faustoferri R C, Hubbard C J, Baldeck J D, Wolf A S, MacGilvray M E, Rosalen P L, Scott-Anne K, Santiago B, Gopal S, Payne J, Marquis R E
Abstract excerpt
A collection of tagged deletion mutant strains was created in Streptococcus mutans UA159 to facilitate investigation of the aciduric capability of this oral pathogen. Gene-specific barcoded deletions were attempted in 1432 open reading frames (representing 73% of the genome), and resulted in the isolation of 1112 strains (56% coverage) carrying deletions in distinct non-essential genes. As S. mutans virulence is...
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