Article
Efficient and Inducible Use of Artificial MicroRNAs in Marchantia polymorpha.
Plant & cell physiology - 1 Feb 2016
Flores-Sandoval Eduardo, Dierschke Tom, Fisher Tom J, Bowman John L
Abstract excerpt
We describe the efficient use of artificial microRNAs (amiRs) in Marchantia polymorpha using both endogenous and heterologous primary microRNA (pri-miR) hairpin backbones. Targeting of two transcription factor genes, MpARF1 and MpRR-B, mediating different hormonal responses, demonstrated that amiRs can create specific and reproducible physiological and morphological defects, facilitating interpretation of gene...
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