Article
ISO 17025 validation of a next-generation sequencing assay for relationship testing.
Electrophoresis - 1 Oct 2016
Buchard Anders, Kampmann Marie-Louise, Poulsen Lena, Børsting Claus, Morling Niels
Abstract excerpt
The HID-Ion AmpliSeq™ Identity Panel is a next-generation sequencing assay with 90 autosomal and 34 Y-chromosome SNPs that are amplified in one PCR step and subsequently sequenced using the Ion Personal Genome Machine (Ion PGM™) System. This assay was validated for relationship testing in our ISO 17025 accredited laboratory in 2015. Here, the essential parts of the validation report submitted to the Danish...
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