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DhITACT: DNA Hydrogel Formation by Isothermal Amplification of Complementary Target in Fluidic Channels.

Advanced materials (Deerfield Beach, Fla.) - 17 Jun 2015

Lee Ho Yeon, Jeong Hansaem, Jung Il Young, Jang Bora, Seo Young Chang, Lee Haeshin, Lee Hyukjin

Abstract excerpt

DNA hydrogel formation by isothermal amplification of complementary targets in microfluidic channels (DhITACT) is a new platform for rapid and accurate detection of infectious pathogens. DNA hydrogel is formed in situ within microfluidic channels by the isothermal rolling circle amplification process upon the selective binding of target strands from the biological fluid. Once the volume of DNA hydrogel...

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PMID: 25946166
DOI: 10.1002/adma.201500414

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