Article
TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea).
BMC genomics - 7 Mar 2015
Guo Yufang, Abernathy Brian, Zeng Yajuan, Ozias-Akins Peggy
Abstract excerpt
BACKGROUND: Targeting Induced Local Lesions in Genomes (TILLING) is a powerful reverse genetics approach for functional genomics studies. We used high-throughput sequencing, combined with a two-dimensional pooling strategy, with either minimum read percentage with non-reference nucleotide or minimum variance multiplier as mutation prediction parameters, to detect genes related to abiotic and biotic stress...
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