Article
A common late-stage intermediate in catalysis by 2-hydroxyethyl-phosphonate dioxygenase and methylphosphonate synthase.
Journal of the American Chemical Society - 11 Mar 2015
Peck Spencer C, Chekan Jonathan R, Ulrich Emily C, Nair Satish K, van der Donk Wilfred A
Abstract excerpt
2-Hydroxyethylphosphonate dioxygenase (HEPD) and methylphosphonate synthase (MPnS) are nonheme iron oxygenases that both catalyze the carbon-carbon bond cleavage of 2-hydroxyethylphosphonate but generate different products. Substrate labeling experiments led to a mechanistic hypothesis in which the fate of a common intermediate determined product identity. We report here the generation of a bifunctional mutant of...
Topics
- Catalysis
- Catalytic Domain
- Crystallography, X-Ray
- Dioxygenases
- Glutamic Acid
- Histidine
- Magnetic Resonance Spectroscopy
- Mutation
- Organophosphonates
- Organophosphorus Compounds
- Oxygenases
- Protein Conformation