Article
Rapid identification of medically important Candida isolates using high resolution melting analysis.
PloS one - 1 Jan 2015
Nemcova Eva, Cernochova Michaela, Ruzicka Filip, Malisova Barbora, Freiberger Tomas, Nemec Petr
Abstract excerpt
An increasing trend in non albicans infections and various susceptibility patterns to antifungal agents implies a requirement for the quick and reliable identification of a number of medically important Candida species. Real-time PCR followed by high resolution melting analysis (HRMA) was developed, tested on 25 reference Candida collection strains and validated on an additional 143 clinical isolates in this...
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