Article
The development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis.
Molecular oral microbiology - 1 Jun 2014
Alnuaimi A D, Wiesenfeld D, O'Brien-Simpson N M, Reynolds E C, Peng B, McCullough M J
Abstract excerpt
Accurate, rapid and economical fungal species identification has been a major aim in mycology. In this study, our goal was to examine the feasibility of a high-resolution melting curve analysis (HRMA) of internal transcribed regions ITS1 and ITS2 in ribosomal DNA (rDNA) for a rapid, simple and inexpensive differentiation of eight clinically relevant Candida species (Candida albicans, Candida glabrata, Candida...
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