Article
Quantification of cells with specific phenotypes II: determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti-CD4 FITC antibody.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Mar 2015
Wang L, Stebbings R, Gaigalas A K, Sutherland J, Kammel M, John M, Roemer B, Kuhne M, Schneider R J, Braun M, Engel A, Dikshit D, Abbasi F, Marti G E, Sassi M, Revel L, Kim S K, Baradez M, Lekishvili T, Marshall D, Whitby L, Jing W, Ost V, Vonsky M, Neukammer J
Abstract excerpt
This report focuses on the characterization of CD4 expression level in terms of equivalent number of reference fluorophores (ERF). Twelve different flow cytometer platforms across sixteen laboratories were utilized in this study. As a first step the participants were asked to calibrate the fluore...
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