Monitoring and improving the sensitivity of dengue nested RT-PCR used in longitudinal surveillance in Thailand.

doi:10.1016/j.jcv.2014.12.009

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Article

Monitoring and improving the sensitivity of dengue nested RT-PCR used in longitudinal surveillance in Thailand.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Feb 2015

Klungthong Chonticha, Manasatienkij Wudtichai, Phonpakobsin Thipwipha, Chinnawirotpisan Piyawan, Rodpradit Prinyada, Hussem Kittinun, Thaisomboonsuk Butsaya, Ong-ajchaowlerd Prapapun, Nisalak Ananda, Kalayanarooj Siripen, Buddhari Darunee, Gibbons Robert V, Jarman Richard G, Yoon In-Kyu, Fernandez Stefan

Abstract excerpt

BACKGROUND: AFRIMS longitudinal dengue surveillance in Thailand depends on the nested RT-PCR and the dengue IgM/IgG ELISA. OBJECTIVE: To examine and improve the sensitivity of the nested RT-PCR using a panel of archived samples collected during dengue surveillance. STUDY DESIGN: A retrospective analysis of 16,454 dengue IgM/IgG ELISA positive cases collected between 2000 and 2013 was done to investigate the...

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PMID: 25600599
DOI: 10.1016/j.jcv.2014.12.009

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