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Rapid identification of dengue virus serotypes by using polymerase chain reaction

1 Oct 1991

Abstract excerpt

Four primer pairs were selected on the basis of the published sequence data of four dengue virus serotypes so that each unique target sequence size could be amplified for each serotype by polymerase chain reaction. The procedure consists of (i) RNA preparation, (ii) reverse transcription, and (iii) polymerase chain reaction, all of which could be completed within 2 h in a single tube for each specimen. The...

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PMID: 1682341
DOI: 10.1128/jcm.29.10.2107-2110.1991

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