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Article

AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping.

Scientific reports - 3 Dec 2014

Xia Zhiqiang, Zou Meiling, Zhang Shengkui, Feng Binxiao, Wang Wenquan

Abstract excerpt

We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaII) and a next-generation sequencing platform. Two sets of 85 adapter pairs were developed to concurrently identify SNPs, indels and methylation sites...

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Identifiers

PMID: 25466435
DOI: 10.1038/srep07300
PMCID: PMC4252907

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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