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Variola Virus-Specific Diagnostic Assays: Characterization, Sensitivity, and Specificity

12 Feb 2015

Abstract excerpt

A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.

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PMID: 25673790
DOI: 10.1128/jcm.03613-14

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