Article
iTRAQ-based quantitative proteome and phosphoprotein characterization reveals the central metabolism changes involved in wheat grain development.
BMC genomics - 27 Nov 2014
Ma Chaoying, Zhou Jianwen, Chen Guanxing, Bian Yanwei, Lv Dongwen, Li Xiaohui, Wang Zhimin, Yan Yueming
Abstract excerpt
BACKGROUND: Wheat (Triticum aestivum L.) is an economically important grain crop. Two-dimensional gel-based approaches are limited by the low identification rate of proteins and lack of accurate protein quantitation. The recently developed isobaric tag for relative and absolute quantitation (iTRAQ) method allows sensitive and accurate protein quantification. Here, we performed the first iTRAQ-based quantitative...
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