Article
Stable phenotype of B-cell subsets following cryopreservation and thawing of normal human lymphocytes stored in a tissue biobank.
Cytometry. Part B, Clinical cytometry - 1 Jan 2015
Rasmussen Simon Mylius, Bilgrau Anders Ellern, Schmitz Alexander, Falgreen Steffen, Bergkvist Kim Steve, Tramm Anette Mai, Baech John, Jacobsen Chris Ladefoged, Gaihede Michael, Kjeldsen Malene Krag, Bødker Julie Støve, Dybkaer Karen, Bøgsted Martin, Johnsen Hans Erik
Abstract excerpt
BACKGROUND: Cryopreservation is an acknowledged procedure to store vital cells for future biomarker analyses. Few studies, however, have analyzed the impact of the cryopreservation on phenotyping. METHODS: We have performed a controlled comparison of cryopreserved and fresh cellular aliquots prepared from individual healthy donors. We studied circulating B-cell subset membrane markers and global gene expression,...
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