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Article

Development of an improved mammalian overexpression method for human CD62L.

Protein expression and purification - 1 Jan 2015

Brown Haley A, Roth Gwynne, Holzapfel Genevieve, Shen Sarek, Rahbari Kate, Ireland Joanna, Zou Zhongcheng, Sun Peter D

Abstract excerpt

We have previously developed a glutamine synthetase (GS)-based mammalian recombinant protein expression system that is capable of producing 5-30mg/L recombinant proteins. The over expression is based on multiple rounds of target gene amplification driven by methionine sulfoximine (MSX), an inhibi...

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Identifiers

PMID: 25286402
DOI: 10.1016/j.pep.2014.09.018
PMCID: PMC4385751

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25286402-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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