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Article

Development of a rapid high-throughput method for high-resolution melting analysis for routine detection and genotyping of noroviruses.

Journal of clinical microbiology - 1 Feb 2009

Tajiri-Utagawa Etsuko, Hara Masayuki, Takahashi Kuniaki, Watanabe Mayumi, Wakita Takaji

Abstract excerpt

We developed a simple, rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a difference plot. The capsid gene was amplified by real-time RT-PCR in the presence of ResoLight HRM dye, a saturating DNA dye. Following optimization of the HRM assay conditions, the major norovirus genotypes were...

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Identifiers

PMID: 19073870
DOI: 10.1128/JCM.01247-08
PMCID: PMC2643702

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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