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Article

Transcriptome analysis of the mud crab (Scylla paramamosain) by 454 deep sequencing: assembly, annotation, and marker discovery.

PloS one - 1 Jan 2014

Ma Hongyu, Ma Chunyan, Li Shujuan, Jiang Wei, Li Xincang, Liu Yuexing, Ma Lingbo

Abstract excerpt

In this study, we reported the characterization of the first transcriptome of the mud crab (Scylla paramamosain). Pooled cDNAs of four tissue types from twelve wild individuals were sequenced using the Roche 454 FLX platform. Analysis performed included de novo assembly of transcriptome sequences, functional annotation, and molecular marker discovery. A total of 1,314,101 high quality reads with an average length...

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PMID: 25054331
DOI: 10.1371/journal.pone.0102668
PMCID: PMC4108364

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