Evaluation of a Real-Time PCR Assay for Simultaneous Detection of <i>Kingella kingae</i> and <i>Staphylococcus aureus</i> from Synovial Fluid in Suspected Septic Arthritis

Abstract excerpt

Direct plating of synovial fluid (SF) on agar-based media often fails to identify pathogens in septic arthritis (SA). We developed a PCR assay for the simultaneous detection of Kingella kingae and Staphylococcus aureus from SF to evaluate molecular detection in SF and to estimate the incidence of K. kingae in SA in North America. The assay was based on detection of the cpn60 gene of K. kingae and the spa gene of...

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