Effect of non-native helix destabilization on the folding of equine β-lactoglobulin.

Abstract excerpt

β-lactoglobulin forms a non-native α-helix during an early stage of folding. To address the role of the non-native structure in the folding process, we designed several mutants of equine β-lactoglobulin with reduced helical propensity in the non-native helix region. One of them, A123T, showed a similar structure to that of the wild-type protein; its folding kinetics was investigated by stopped-flow circular...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Circular Dichroism
• Share to Horses
• Share to Kinetics