Article
Mutation of praR in Rhizobium leguminosarum enhances root biofilms, improving nodulation competitiveness by increased expression of attachment proteins.
Molecular microbiology - 1 Aug 2014
Frederix Marijke, Edwards Anne, Swiderska Anna, Stanger Andrew, Karunakaran Ramakrishnan, Williams Alan, Abbruscato Pamela, Sanchez-Contreras Maria, Poole Philip S, Downie J Allan
Abstract excerpt
In Rhizobium leguminosarum bv. viciae, quorum-sensing is regulated by CinR, which induces the cinIS operon. CinI synthesizes an AHL, whereas CinS inactivates PraR, a repressor. Mutation of praR enhanced biofilms in vitro. We developed a light (lux)-dependent assay of rhizobial attachment to roots and demonstrated that mutation of praR increased biofilms on pea roots. The praR mutant out-competed wild-type for...
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