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Article

Rational design, preparation, and characterization of a therapeutic enzyme mutant with improved stability and function for cocaine detoxification.

ACS chemical biology - 15 Aug 2014

Fang Lei, Chow K Martin, Hou Shurong, Xue Liu, Chen Xiabin, Rodgers David W, Zheng Fang, Zhan Chang-Guo

Abstract excerpt

Cocaine esterase (CocE) is known as the most efficient natural enzyme for cocaine hydrolysis. The major obstacle to the clinical application of wild-type CocE is the thermoinstability with a half-life of only ∼12 min at 37 °C. The previously designed T172R/G173Q mutant (denoted as enzyme E172-173) with an improved in vitro half-life of ∼6 h at 37 °C is currently in clinical trial Phase II for cocaine overdose...

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Identifiers

PMID: 24919140
DOI: 10.1021/cb500257s
PMCID: PMC4136690

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