Article
Predicted Michaelis-Menten complexes of cocaine-butyrylcholinesterase. Engineering effective butyrylcholinesterase mutants for cocaine detoxication.
The Journal of biological chemistry - 23 Mar 2001
Sun H, El Yazal J, Lockridge O, Schopfer L M, Brimijoin S, Pang Y P
Abstract excerpt
Butyrylcholinesterase (BChE) is important in cocaine metabolism, but it hydrolyzes (-)-cocaine only one-two thousandth as fast as the unnatural (+)-stereoisomer. A starting point in engineering BChE mutants that rapidly clear cocaine from the bloodstream, for overdose treatment, is to elucidate structural factors underlying the stereochemical difference in catalysis. Here, we report two three-dimensional...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.