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Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys

8 May 2014

Abstract excerpt

BACKGROUND: Nested PCR is considered a sensitive and specific method for detecting malaria parasites and is especially useful in epidemiological surveys. However, the preparation of DNA templates for PCR is often time-consuming and costly. METHODS: A simplified PCR method was developed to directly use a small blood filter paper square (2 × 2 mm) as the DNA template after treatment with saponin. This filter...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 24884761
DOI: 10.1186/1475-2875-13-175

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