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Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples

19 Aug 2011

Abstract excerpt

BACKGROUND: Real-time PCR is a sensitive and specific method for the analysis of Plasmodium DNA. However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products. METHODS: Reagents designed to specifically overcome PCR inhibition and quenching of fluorescence were evaluated for real-time...

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PMID: 21851640
DOI: 10.1186/1475-2875-10-244

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