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Article

Common Contaminants in Next-Generation Sequencing That Hinder Discovery of Low-Abundance Microbes

16 May 2014

Abstract excerpt

Unbiased high-throughput sequencing of whole metagenome shotgun DNA libraries is a promising new approach to identifying microbes in clinical specimens, which, unlike other techniques, is not limited to known sequences. Unlike most sequencing applications, it is highly sensitive to laboratory contaminants as these will appear to originate from the clinical specimens. To assess the extent and diversity of sequence...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 24837716
DOI: 10.1371/journal.pone.0097876

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