Article
Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.
PLoS neglected tropical diseases - 1 May 2014
Franz Alexander W E, Sanchez-Vargas Irma, Raban Robyn R, Black William C, James Anthony A, Olson Ken E
Abstract excerpt
In 2006, we reported a mariner (Mos1)-transformed Aedes aegypti line, Carb77, which was highly resistant to dengue-2 virus (DENV2). Carb77 mosquitoes expressed a DENV2-specific inverted-repeat (IR) RNA in midgut epithelial cells after ingesting an infectious bloodmeal. The IR-RNA formed double-stranded DENV2-derived RNA, initiating an intracellular antiviral RNA interference (RNAi) response. However, Carb77...
Topics
- Aedes
- Animals
- Base Sequence
- Dengue Virus
- Gene Frequency
- Genetic Fitness
- Genomic Instability
- Insect Vectors
- Molecular Biology
- Molecular Sequence Data
- Phenotype
- RNA Interference
- Transgenes