Article
[Gene diversity of the bacterial 48 family glycoside hydrolase (GH48) in rumen environment].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Jan 2014
Li Jingting, Su Xiaoyun, Tian Yan, Dong Zhiyang, Hu Songnian, Huang Li, Dai Xin
Abstract excerpt
OBJECTIVE: The gene diversity of the bacterial 48 family glycoside hydrolase (GH48) in rumen environment was studied and new gene resources for efficient cellulose degradation were provided. METHODS: A pair of gh48 degenerate primers was designed through sequences alignment of the gh48 gene sequences from ruminal Ruminococcus. The total DNA and RNA were extracted from two rumen samples and cDNA was synthetized...
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