Article
Genome-wide identification of AR-regulated genes translated in Sertoli cells in vivo using the RiboTag approach.
Molecular endocrinology (Baltimore, Md.) - 1 Apr 2014
De Gendt Karel, Verhoeven Guido, Amieux Paul S, Wilkinson Miles F
Abstract excerpt
An understanding of the molecular mechanisms by which androgens drive spermatogenesis has been thwarted by the fact that few consistent androgen receptor (AR) target genes have been identified. Here, we addressed this issue using next-generation sequencing coupled with the RiboTag approach, which purifies translated mRNAs expressed in cells that express cyclic recombinase (CRE). Using RiboTag mice expressing CRE...
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