Article
Mutant OmpF porins of Yersinia pseudotuberculosis with deletions of external loops: structure-functional and immunochemical properties.
Biochemical and biophysical research communications - 7 Mar 2014
Sidorova O V, Khomenko V A, Portnyagina O Yu, Likhatskaya G N, Vakorina T I, Kim N Yu, Chistyulin D K, Solov'eva T F, Novikova O D
Abstract excerpt
Recombinant mutant OmpF porins from Yersinia pseudotuberculosis outer membrane were obtained using site-directed mutagenesis. Here we used four OmpF mutants where single extracellular loops L1, L4, L6, and L8 were deleted one at a time. The proteins were expressed in Escherichia coli at levels co...
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