Article
Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
PloS one - 1 Jan 2013
Nisa-Martínez Rafael, Laporte Philippe, Jiménez-Zurdo José Ignacio, Frugier Florian, Crespi Martin, Toro Nicolás
Abstract excerpt
Some bacterial group II introns are widely used for genetic engineering in bacteria, because they can be reprogrammed to insert into the desired DNA target sites. There is considerable interest in developing this group II intron gene targeting technology for use in eukaryotes, but nuclear genomes present several obstacles to the use of this approach. The nuclear genomes of eukaryotes do not contain group II...
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