Article
An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation.
The Plant journal : for cell and molecular biology - 1 Feb 2014
Bai Chao, Rivera Sol M, Medina Vicente, Alves Rui, Vilaprinyo Ester, Sorribas Albert, Canela Ramon, Capell Teresa, Sandmann Gerhard, Christou Paul, Zhu Changfu
Abstract excerpt
We have developed an assay based on rice embryogenic callus for rapid functional characterization of metabolic genes. We validated the assay using a selection of well-characterized genes with known functions in the carotenoid biosynthesis pathway, allowing rapid visual screening of callus phenotypes based on tissue color. We then used the system to identify the functions of two uncharacterized genes: a chemically...
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