Article
Analysis of Y chromosome haplogroups in Japanese population using short amplicons and its application in forensic analysis.
Legal medicine (Tokyo, Japan) - 1 Jan 2014
Harayama Yuta, Kamei Sayako, Sato Noriko, Hayashi Tokutaro, Shiozaki Tetsuya, Ota Masao, Asamura Hideki
Abstract excerpt
We designed three mini multiplex PCR systems using single-base extension reactions to identify Japanese Y chromosome haplogroups. We selected a group of 22 Y chromosome single nucleotide polymorphisms (SNPs) from the haplogroups most commonly reported in East Asia. To make the systems more useful in analyzing degraded DNA samples, we designed primers to render amplicons of ≤ 150 bp. Applying these systems, we...
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