Article
Identification accuracy and diversity reproducibility associated with internal transcribed spacer-based fungal taxonomic library preparation.
Environmental microbiology - 1 Sept 2014
Yamamoto Naomichi, Dannemiller Karen C, Bibby Kyle, Peccia Jordan
Abstract excerpt
This study investigated analytical parameters that are inherently relevant to identifying and quantifying fungal communities based on polymerase chain reaction amplicons. Specifically, we evaluated the accuracy of the BLASTn-based identification for internal transcribed spacer (ITS) sequences generated from pure cultures, and quantified the reproducibility of relative abundances as well as α and β diversity...
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