Article
The limitations of pulsed-field gel electrophoresis for analysis of Yersinia enterocolitica isolates.
Zoonoses and public health - 1 Sept 2014
Gilpin B J, Robson B, Lin S, Hudson J A, Weaver L, Dufour M, Strydom H
Abstract excerpt
This study describes the analysis of 432 isolates of Yersinia enterocolitica by pulsed-field gel electrophoresis (PFGE). PFGE had a high level of discrimination with biotype 1A isolates (Simpson's Diversity Index 0.997), but with the clinically important biotypes 2, 3 and 4, the discriminatory ability of PFGE was so low as to severely limit its usefulness (DI <0.6). For biotypes 2, 3 and 4, 79% or more of...
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