Article
Acyl-lipid thioesterase1-4 from Arabidopsis thaliana form a novel family of fatty acyl-acyl carrier protein thioesterases with divergent expression patterns and substrate specificities.
Plant molecular biology - 1 Mar 2014
Pulsifer Ian P, Lowe Christine, Narayaran Swara A, Busuttil Alia S, Vishwanath Sollapura J, Domergue Frédéric, Rowland Owen
Abstract excerpt
Hydrolysis of fatty acyl thioester bonds by thioesterases to produce free fatty acids is important for dictating the diversity of lipid metabolites produced in plants. We have characterized a four-member family of fatty acyl thioesterases from Arabidopsis thaliana, which we have called acyl-lipid thioesterase1 (ALT1), ALT2, ALT3, and ALT4. The ALTs belong to the Hotdog fold superfamily of thioesterases. ALT-like...
Topics
- Acyl Carrier Protein
- Amino Acid Sequence
- Arabidopsis
- Arabidopsis Proteins
- Fatty Acids
- Green Fluorescent Proteins
- In Situ Hybridization
- Isoenzymes
- Microscopy, Confocal
- Molecular Sequence Data
- Multigene Family
- Mutation