Article
Improved stearate phenotype in transgenic canola expressing a modified acyl-acyl carrier protein thioesterase.
Nature biotechnology - 1 Jun 1999
Facciotti M T, Bertain P B, Yuan L
Abstract excerpt
The engineering of crops for selected fatty acid production is one of the major goals of plant biotechnology. The Garm FatA1, an acyl-acyl carrier protein (ACP) thioesterase isolated from Garcinia mangostana, generates an elevated stearate (18:0) phenotype in transgenic Brassica plants. By site-directed mutagenesis, we generated seven mutants that showed up to a 13-fold increase in specific enzyme activity toward...
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