Article
Microsatellite markers from the Ion Torrent: a multi-species contrast to 454 shotgun sequencing.
Molecular ecology resources - 1 May 2014
Elliott Carole P, Enright Neal J, Allcock Richard J N, Gardner Michael G, Meglécz Emese, Anthony Janet, Krauss Siegfried L
Abstract excerpt
The development and screening of microsatellite markers have been accelerated by next-generation sequencing (NGS) technology and in particular GS-FLX pyro-sequencing (454). More recent platforms such as the PGM semiconductor sequencer (Ion Torrent) offer potential benefits such as dramatic reductions in cost, but to date have not been well utilized. Here, we critically compare the advantages and disadvantages of...
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