Article
Depletion of the xynB2 gene upregulates β-xylosidase expression in C. crescentus.
Applied biochemistry and biotechnology - 1 Jan 2014
Corrêa Juliana Moço, Mingori Moara Rodrigues, Gandra Rinaldo Ferreira, Loth Eduardo Alexandre, Seixas Flávio Augusto Vicente, Simão Rita de Cássia Garcia
Abstract excerpt
Caulobacter crescentus is able to express several enzymes involved in the utilization of lignocellulosic biomasses. Five genes, xynB1-5, that encode β-xylosidases are present in the genome of this bacterium. In this study, the xynB2 gene, which encodes β-xylosidase II (CCNA_02442), was cloned under the control of the PxylX promoter to generate the O-xynB2 strain, which overexpresses the enzyme in the presence of...
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