Characterization of Escherichia coli thioredoxins with altered active site residues.

doi:10.1021/bi00467a016

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Article

Characterization of Escherichia coli thioredoxins with altered active site residues.

Biochemistry - 17 Apr 1990

Gleason F K, Lim C J, Gerami-Nejad M, Fuchs J A

Abstract excerpt

Escherichia coli thioredoxin is a small disulfide-containing redox protein with the active site sequence Cys-Gly-Pro-Cys-Lys. Mutations were made in this region of the thioredoxin gene and the mutant proteins expressed in E. coli strains lacking thioredoxin. Mutant proteins with a 17-membered or...

Topics

Amino Acid Sequence
Bacterial Proteins
Binding Sites
Escherichia coli
Kinetics
Models, Biological
Molecular Sequence Data
Mutation
Protein Conformation
Spectrometry, Fluorescence
Thioredoxin-Disulfide Reductase
Thioredoxins

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