Localization of enterobacterial common antigen immunoreactivity in the ribosomal cytoplasm of Escherichia coli cells cryosubstituted and embedded at low temperature.

doi:10.1128/jb.172.2.1106-1113.1990

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Article

Localization of enterobacterial common antigen immunoreactivity in the ribosomal cytoplasm of Escherichia coli cells cryosubstituted and embedded at low temperature.

Journal of bacteriology - 1 Feb 1990

Acker G, Kammerer C

Abstract excerpt

The application of two on-section immunogold labeling techniques, the Lowicryl K4M (progressive lowering of temperature) procedure and the cryosection technique of Tokuyasu, in a previous work to study the topology of enterobacterial common antigen (ECA) biosynthesis revealed the presence of labe...

Topics

Antigens, Bacterial
Cytoplasm
Enterobacteriaceae
Escherichia coli
Freezing
Immunohistochemistry
Microscopy, Electron
Mutation
Ribosomes
Tunicamycin

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