Article
Eight nucleotide substitutions inhibit splicing to HPV-16 3'-splice site SA3358 and reduce the efficiency by which HPV-16 increases the life span of primary human keratinocytes.
PloS one - 1 Jan 2013
Li Xiaoze, Johansson Cecilia, Cardoso Palacios Carlos, Mossberg Anki, Dhanjal Soniya, Bergvall Monika, Schwartz Stefan
Abstract excerpt
The most commonly used 3'-splice site on the human papillomavirus type 16 (HPV-16) genome named SA3358 is used to produce HPV-16 early mRNAs encoding E4, E5, E6 and E7, and late mRNAs encoding L1 and L2. We have previously shown that SA3358 is suboptimal and is totally dependent on a downstream splicing enhancer containingmultiple potential ASF/SF2 binding sites. Here weshow that only one of the predicted ASF/SF2...
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