Emitting state of 5-hydroxyindole, 5-hydroxytryptophan, and 5-hydroxytryptophan incorporated in proteins.

Abstract excerpt

5-Hydroxy-L-tryptophan (5HW) has been biosynthetically incorporated in many proteins to facilitate their characterization using fluorescence spectroscopy. An attractive feature of this tryptophan analogue is its absorbance at 310-320 nm, allowing its specific excitation in a Trp background. The red-shift in absorbance upon introduction of a hydroxyl group at the 5-position of Trp or indole was found to be due to...

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