Article
Evaluation of a reverse line blot assay for genotyping common human rotaviruses.
Journal of virological methods - 1 Nov 2013
Duizer Erwin, van der Veer Bas, de Bruin Erwin, Zeller Mark, Heylen Elisabeth, van Ranst Marc, Matthijnssens Jelle, Koopmans Marion, Vennema Harry
Abstract excerpt
To allow high-throughput genotyping of group A rotaviruses (RVA) in a routine surveillance setting, we developed a reverse line blotting method for the determination of the most common human RVA G- and P-genotypes: G1-G4, G9, G12, P[4], P[6] and P[8]. Using the reverse line blotting method on 951 clinical RVA positive feces samples, in 905 (95%) of the samples the G-genotyping yielded a result while in 945 (99%)...
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